BLOC1S1: An Unexpected Regulator of Th2 Cell-driven Inflammatory Responses

Abstract BLOC1S1 is an adaptor protein that modulates endosome-lysosome function as a component of numerous multiprotein complexes. We postulated its modulation in CD4 +T cells may help us uncover the role biology adaptive immunity. In primary cre-recombinase knockout resulted preferential induction Th2 compared to Th1 and Th17 cytokine production following T cell receptor activation. As atopic dermatitis allergic inflammatory skin disease characterized by type 2 cytokines we evaluated effect KO vs controls calcipotriol-induced mice. The absence exhibited exacerbated accompanied with significantly elevated plasma IgE, eosinophilia, responses, pathology relative wild-type transcription factor Gata-3 expressed but not plays critical differentiation dermatitis. deficient showed enhanced nuclear kB (NF-kB), Gata3 LC3 positive autophagosomes. Inhibition NF-kB activity prevented expression, autophagosomes −/− cells. This mouse model will now give platform explore endo-lysosomal polarization NHLBI Division Intramural Research (MNS-ZIA-HL005102)